In vivo and in vitro studies of the combined toxic effect of selected organic solvents

The aim of the project was to assess the combined effect of selected organic solvents in vivo and in vitro.Within the project, the following were assessed:- in vivo, the effect of ethanol or toluene on the hemolytic effect of methoxyethanol (ME), ethoxyethanol (EE) and, additionally, butoeyethanole (BE) in repeated exposure of animals;- in vitro, the cytotoxic effect of methoxyethanol metabolites (methoxyacetic acid, MAA) or ethoxyethanol (ethoxyacetic acid, EAA) with ethanol or toluene (or their metabolites) on two cell lines of Chinese hamster ovary fibroblasts (CH)-9) and human non-small cell lung cancer. (A549).On the basis of the tests it was determined that the protective effect of ethanol and toluene on the hematological parameters in animals in repetitive exposure to ethylene glycol ethers (EGAEs) can result from a greater affinity of enzymes that metabolize them to ethanol or toluene than to the studied ethers.